有关参青方改善溃疡性结肠炎“里急后重”症状作用机制的研究

　　第一作者：戴彦成，通讯作者：唐志鹏

　　内容介绍：溃疡性结肠炎患者常有里急后重的症状，表现为：自觉腹内拘急，疼痛不舒，便意急迫，但肛门重坠，便出不爽。中药参青方可以通过修复肠道粘膜损伤，修复Cajal间质细胞从而发挥其调节肠道动力，改善“里急后重”的症状。上海龙华医院脾胃病科戴彦成

　　Influence of Shenqing Recipe on Morphology and Quantity of Colonic Interstitial Cells of Cajal in Trinitrobenzene Sulfonic Acid Induced Rat Colitis△

　　Yan-cheng Dai, Zhi-peng Tang*, Zhen-nan Wang,

　　Ya-li Zhang, and Xin-ying He

　　Department of Gastroenterology, Longhua Hospital; Institute of Digestive Disease,

　　Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China

　　Key words: Shenqing Recipe; trinitrobenzene sulfonic acid; colitis; c-kit; interstitial

　　cells of Cajal

　　Objective To observe the influence of Shenqing Recipe (SQR), a kind of Traditional Chinese Medi-cine, on the morphology and quantity of colonic interstitial cells of Cajal (ICC) in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis, and to investigate the possible mechanism of SQR in regulating intestinal dynamics.

　　Methods Sixty rats were randomly divided into normal control, model Ⅰ, model Ⅱ, mesalazine, and high-dose, and low-dose SQR groups with 10 rats in each group. TNBS (10 mg) dissolved in 50% etha-nol was instilled into the lumen of the rat colon of the latter five groups to induce colitis. On the 4th day af-ter administration of TNBS, each treatment group was administered one of the following formulations by enteroclysis gavage once a day for 7 days: 600 mg・kg-1・d-1 mesalazine, 2.4 g・kg-1・d-1 SQR, and 1.2 g・kg-1・d-1 SQR. Model Ⅱ rats received normal saline solution. After 7 days colonic samples were collected. While the colonic samples of model Ⅰ group were collected on the 3rd day after TNBS administered. Ultrastructure of ICC in the damaged colonic tissues was observed with transmission electron microscope. Expression of c-kit protein in colonic tissue was determined by immunohistochemical staining and Western blot.

　　Results The ultrastructure of colonic ICC in the rat model of TNBS-induced colitis showed a severe injury, and administration of SQR or mesalazine reduced the severity of injury. Similarly, the expression of c-kit protein of TNBS-induced colitis rat model was significantly decreased compared with the normal control group (P<0.05). Treatment with SQR or mesalazine significantly increased the expression of c-kit protein com-pared with the administration of control formulations (P<0.05), especially the high-dose SQR group.

　　Conclusion SQR could alleviate and repair the injured ICC, and improve its quantity, which might be involved in regulating intestinal motility.

　　Chin Med Sci J 2011; 26(1):43-48